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We first discuss methods that allow the observation of morphological changes during cell death Understanding how a compound can activate the various pathways of cell death is essential for determining the acute, subacute, and chronic effects of drugs and chemical toxicants. We then describe and compare the array of different biochemical assays for each specific type of cell death.

Cell death assays are important across research and medicine These tips are informed by our own research, the problems we have encountered and the mistakes we have made, and our efforts to measure cell death as carefully as possible. In drug discovery and development, these assays evaluate the efficacy and potential toxicity of new therapeutic compounds.

As cell death can occur by several diferent pathways, including apoptosis, necrosis, autophagy, and necroptosis, some of which share characteristics, you may need to examine multiple apoptosis markers to confirm that this is the mechanism of cell death in your experimental system.

Here, we introduce a range of assays for measuring cell death in cultured cells, and we outline basic techniques for distinguishing healthy cells from apoptotic or necrotic cells—the two most common forms of cell death. Cell death is an important process in the body Discover how we can achieve greater analysis of cell death to benefit and improve research initiatives. To study cell death, scientists use cell death assays, which help identify and quantify dying cells, determine the type of cell death, and assess drug responses

In this article, we will explore the types of cell death, different cell death assays, their applications, and advancements in the field Promoting appropriate cell life and death is a key part of tissue engineering When cells are put into contact with a biomaterial (or into any novel culture condition), their viability may be affected Some materials are cytotoxic, i.e., deadly to cells.

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